Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FBXO44

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231
cell line
MDA-MB-231
antibody
FBXO44 antibody (Sigma-Aldrich no. HPA003363)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For FBXO44, cells were crosslined and sonicated. For H3K9me3 ChIP, native ChIP was performed. Libraries were made using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
18958642
Reads aligned (%)
95.1
Duplicates removed (%)
26.1
Number of peaks
545 (qval < 1E-05)

hg19

Number of total reads
18958642
Reads aligned (%)
94.4
Duplicates removed (%)
27.5
Number of peaks
565 (qval < 1E-05)

Base call quality data from DBCLS SRA