Antigen

Antigen Class

TFs and others

Antigen

FBXO44

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MDA-MB-231

cell line

MDA-MB-231

antibody

FBXO44 antibody (Sigma-Aldrich no. HPA003363)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For FBXO44, cells were crosslined and sonicated. For H3K9me3 ChIP, native ChIP was performed. Libraries were made using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

18958642

Reads aligned (%)

95.1

Duplicates removed (%)

26.1

Number of peaks

545 (qval < 1E-05)

hg19

Number of total reads

18958642

Reads aligned (%)

94.4

Duplicates removed (%)

27.5

Number of peaks

565 (qval < 1E-05)